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Conventional cytogenetics and fluorescence in situ hybridization as methods for detecting IgH gene rearrangements
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2006.04.001
- Key Word:
- 细胞遗传学;荧光原位杂交;免疫球蛋白重链基因重排
Abstract： 目的 比较常规细胞遗传学(CC),间期荧光原位杂交(FISH)技术及连续R显带后FISH检测免疫球蛋白重链(IgH)基因重排的应用价值.方法 应用常规细胞遗传学及间期FISH分析血液系统肿瘤患者43例.结果 43例患者中14q32+/IgH+患者19例,14q32-/IgH+患者2例(4.7%),14q32+/IgH-患者3例(7.0%),14q32-/IgH-患者19例.部分病例CC与间期FISH方法检测14q32/IgH基因重排得到了不一致的结果.对5例患者进行连续R显带后FISH分析,对照核型和FISH图,能清楚地看到IgH基因易位涉及的染色体.结论 间期FISH可以提高14q32/IgH重排检出率,R显带后FISH可以帮助识别与14q32易位的伙伴染色体.
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