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Effect on minimal residual leukemia in mice model by interlukin-2 and matrine
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1009-9921.2006.01.002
- Key Word:
- 白细胞介素-2;苦参碱;白血病
Abstract： 目的研究微小残留白血病的治疗新方法.方法采用重组人白细胞介素-2(rhIL-2)和苦参碱单独或联合治疗小鼠多药耐药微小残留白血病.DBA小鼠接种P388/VCR-G细胞(1×106/只)后第3天,用环磷酰胺(Cy)35 mg/kg一次腹腔注射,24 h后实验分4组.对照组:腹腔注射生理盐水0.2ml/次,2次/d,连续5 d.rhIL-2组:腹腔注射rhIL-2 1×105U/次,2次/d,连续5 d.苦参碱组:腹腔注射苦参碱25mg/kg,1次/d,连续5 d.rhIL-2+苦参碱组:腹腔注射rhIL-2 1×105U/次,2次/d,连续5 d;苦参碱25 mg/kg,1次/d,连续5 d.结果对照组小鼠平均生存期(25.2±1.10)d,rhIL-2治疗组平均生存期分别延长至(36.25±1.26)d,按回归方程y天数=-1.98x细胞数+28.61推算,相当于杀灭了1×103左右的白血病细胞.苦参碱治疗组平均生存期分别延长至(37.8±13.7)d,亦相当于杀灭了1×103左右的白血病细胞.rhIL-2联合苦参碱治疗组平均生存期为(37.0±1.87)d,与单用比较无统计学差异(P＞0.05).结论rhIL-2和苦参碱单独应用均有显著抑制微小残留白血病细胞增生的作用,但两者联合应用无明显协同作用.
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