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A study on the significance of HLA-CW typing in hematological stem cell transplantation
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2005.03.004
- Key Word:
- HLA-CW基因;造血干细胞移植;急性移植物抗宿主病
Abstract： 目的探讨HLA-CW位点基因分型在造血干细胞移植(hematopoicetic stem cell transplantation-HSCT)中的作用,对HLA-CW基因位点相合与不相合造血干细胞移植的效果进行分析.方法采用聚合酶链反应-序列特异性引物扩增(polymerase chain reaction-sequence specific primers,PCR-SSP)方法对HSCT供、受体共42个样本进行HLA-CW位点等位基因分析.结果HLA-CW基因全相合者9例造血重建,存活200 d以上7例,1例存活303d复发,2例存活44 d(死于感染),无病生存率(66.7±3.4)%.1例(11.1%)发生急性移植物抗宿主病(aGVHD),3例(33.3%)发生慢性移植物抗宿主病(cGVHD).HLA-CW基因不相合者12例造血重建,存活200 d以上6例,1例存活105 d复发,6例存活75 d死亡,无病生存率(41.7±5.4)%(P＜0.05).6例(50%)发生aGVHD;5例(41.7%)发生cGVHD(P＞0.05).结论HLA-CW基因不相合导致的aGVHD及相关死亡率明显高于CW基因相合者,显示CW基因不相合也是造成aGVHD发生率增高和影响移植效果的重要因素.
- [1]陈育红,黄晓军,郭乃榄,等.异基因造血干细胞移植后急性移植物抗宿主病的发生及其危险因素的探讨[J].2003,(2).
- [2]M, Takiguchi,I, Nishimura,H, Hayashi,etc.The structure and expression of genes encoding serologically undetected HLA-C locus antigens.[J].1989,143.
- [3]M, Bunce,K I, Welsh.Rapid DNA typing for HLA-C using sequence-specific primers (PCR-SSP): identification of serological and non-serologically defined HLA-C alleles including several new alleles.[J].1994,43(1).
- [4]Sasazuki, Takehiko,Juji, Takeo,Morishima, Yasuo,etc.Effect of Matching of Class I HLA Alleles on Clinical Outcome after Transplantation of Hematopoietic Stem Cells from an Unrelated Donor.[J].1998,339(17).
- [5]Y, Shimizu,R, DeMars.Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line.[J].1989,142(9).