You Position: Home > Paper
The research of the correlation of protein kinase C and the mdr of K562/A02 cells
( views:249, downloads:8 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1009-9921.2005.01.003
- Key Word:
- 蛋白激酶C;多药耐药;粉防己碱;屈洛昔芬;细胞系;K562
Abstract： 目的探讨蛋白激酶C(protein kinase C,PKC)在K562/A02细胞多药耐药的产生及其逆转中的作用.方法用放射免疫法检测了耐药细胞株K562/A02及其敏感株K562的静息PKC活性水平,并观察了柔红霉素(daunomycin,DNR)以及耐药调节剂粉防己碱(tetrandrine,Tet)、屈洛昔芬(droloxifene,Drol)单独或联合应用后细胞PKC活性的变化.结果静息状态下耐药细胞株K562/A02的PKC活性显著高于敏感株K562,有效调节浓度的Tet、Drol单独作用于K562、K562/A02细胞均可显著下调其PKC活性,联合应用有显著协同性.1μg/mLDNR可显著下调K562细胞的PKC活性,部分下调K562/A02细胞的PKC活性,有效调节浓度的Tet、Drol单独或联合应用均可加强其作用.结论PKC参与K562/A02细胞多药耐药(multidrug resistance,MDR)的形成,Tet、Drol逆转K562/A02细胞的MDR可能与下调其PKC活性有关.
- [1]李杰,许良中,何开玲.甲诺孕酮和屈洛昔芬对K562/A02细胞株多种耐药机制的调节[J].1999,(6).
- [2]陈宝安,董颖,张鹏,等.汉防己甲素联合屈洛昔芬逆转K562/A02细胞多药耐药的研究[J].2002,(12).
- [3]赵春亭,杨纯正,邵晓枫.蛋白激酶C在K562/AO2细胞多药耐药中作用的初步研究[J].1998,(5).
- [4]陈宝安,董颖,张鹏,等.汉防己甲素联合屈洛昔芬逆转K562/A02细胞耐药与诱导凋亡[J].2002,(6).
- [5]马强,张振书,钟世顺,等.PKC对大肠癌耐药LoVo/Adr细胞阿霉素摄入及分布的影响[J].2002,(4).
- [6]R L, Fine,J, Patel,B A, Chabner.Phorbol esters induce multidrug resistance in human breast cancer cells.[J].1988,85.
- [7]G, Bradley,P F, Juranka,V, Ling.Mechanism of multidrug resistance.[J].1988,948.