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DETECTION AND CLINICAL VALUE OF IMMUNOGLOBULIN KAPPA LIGHT CHAIN GENE REARRANGEMENT IN THE B CELL LYMPHOPROLIFERATIVE DISEASES
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2004.05.008
- Key Word:
- 克隆性;kappa;轻链;基因重排;非霍奇金淋巴瘤
Abstract： 目的:评价免疫球蛋白kappa基因重排检测在B淋巴细胞增生性疾病中的价值.方法:选取93例B淋巴细胞增生性疾病及反应性增生标本(其中包括43例甲醛固定,石蜡包埋标本),运用降落PCR方法扩增Ig kappa CDR3区,以异源双链分析鉴别扩增产物以分辨其克隆性.结果:在所有确诊的72例B淋巴细胞增生性疾病中Ig kappa扩增的阳性率为47%,其中14例急性B淋巴细胞白血病中有2例阳性(14%);3例慢性淋巴细胞白血病中有2例阳性(67%);55例B细胞恶性淋巴瘤中有30例阳性(55%).在11例不典型增生的病例中,Igκ单克隆检出率为2/11(18%).10例反应性增生中无一出现单克隆重排.石蜡组织中扩增成功率为32/43(74%).结论:作为一个独立的分子标志,以PCR方法扩增Ig kappa确定B淋巴细胞增生性疾病的克隆性在其临床诊断及生物学特性研究方面是一种有前途的方法,且适用于石蜡标本以进行回顾性研究.
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