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EXPRESSION OF INTERLEUKIN-23 AND INTERLEUKIN-12 IN HUMAN LEUKEMIA CELL LINES
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2004.05.004
- Key Word:
- 白细胞介素-23;白血病细胞系;佛波二酯;EB病毒
Abstract： 目的:检测人白细胞介素-23(IL-23)和白细胞介素-12(IL-12)亚基在各类白血病细胞系中的表达并探讨佛波二酯对IL-23和IL-12亚基表达的调控作用.方法:半定量RT-PCR方法检测13株人白血病细胞系IL-23p19,IL-12p35,IL-12p40亚基的表达.用佛波二酯处理白血病细胞系,不同时间点取样,半定量RT-PCR方法检测上述亚基的表达变化.结果:检测的大多数白血病细胞系均表达p19 mRNA,p19比p35表达更为普遍;而p40仅在EB病毒感染的LCL和J6-1细胞中测到.佛波二酯可以提高EB病毒阳性细胞系中p19,p35和p40的表达水平.结论:p19可能广泛表达于各类血细胞.佛波二酯可上调EB病毒阳性细胞系p19,p35和p40的表达,表明IL-23与IL-12相类似,其在白血病细胞系中的表达可能与EB病毒感染相关.
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