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QUALITATIVE AND QUANTITATIVE CHARACTERIZATION OF FAS (CD95) EXPRESSION AND ITS ROLE IN PRIMARY HUMAN ACUTE LEUKEMIA CELLS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1009-9921.2004.01.002
- Key Word:
- Fas(CD95);细胞凋亡;急性白血病;流式细胞术
Abstract： 目的:研究急性白血病细胞中Fas的定性和定量表达及功能.方法:应用流式细胞术及RT-PCR方法检测急性白血病患者外周血或骨髓中白血病细胞的Fas定性和定量表达,并用AnnexinV染色法检测其功能.结果:急性白血病细胞中Fas蛋白呈单峰状表达,具有不同的密度,并且在不同类型细胞中表现出不同的定量特征;Fas呈定性阴性或定量弱表达的患者中,其全长和剪接体mRNA的表达水平与蛋白的表达水平有关;新分离出来的白血病细胞在体外与pokeweed(PWA)抗原共同培养后,可引起Fas表达水平的上调;新鲜的急性淋巴细胞白血病(ALL)和急性髓系白血病(AML)细胞对于抗FasIgM抗体有抵抗能力,而鬼臼乙叉苷(etoposide,ETP)能够引起所有类型的急性白血病细胞产生凋亡.抗FasIgM抗体和ETP的联合使用并不能产生协同效果.结论:Fas是ALL和AML细胞的一个生物学标志,该研究为探讨细胞毒素制剂和细胞因子治疗作用的机制提供了线索.
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