You Position: Home > Paper
STUDY ON THE ANTI-LEUKEMIA IMMUNE EFFECT INDUCED BY DENDRITIC CELLS DERIVED FROM CORD BLOOD, NORMAL HUMAN PERIPHERAL BLOOD AND CHRONIC MYELOID LEUKEMIA CELLS
( views:256, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1009-9921.2003.06.004
- Key Word:
- 树突状细胞;抗白血病免疫;脐血;慢性粒细胞白血病;健康人外周血
Abstract： 目的:观察脐血、健康人及慢性粒细胞性白血病(CML)患者外周血3种不同来源的树突状细胞(DC)及其抗白血病效应.方法:分离3种来源的单个核细胞(MNC)并在细胞因子(GM-CSF+IL-4+TNF-a)作用下诱导培养DC.观察DC形态,流式细胞技术检测DC免疫表型(CD1a、CD80、CD83、HLA-DR);将所得DC经CML细胞可溶性抗原负载后和其同源T细胞混合诱导抗原特异性CTL,MTT法检测其杀伤活性.结果:3种来源MNC在细胞因子作用下均可获得形态典型的DC,且高表达CD1a、CD80、CD83、HLA-DR,经抗原负载后的DC诱导的CTL对CML靶细胞的杀伤活性也较强.结论:脐血、健康人及CML患者外周血均为理想的DC来源;有较好的诱导CTL杀伤白血病细胞效应;应用DC瘤苗免疫治疗白血病必将成为一种崭新的肿瘤免疫治疗方法,有十分诱人的发展前景.
- [1]王黎明,陈珊珊,刘艳荣,等.慢性髓性白血病来源的树突状细胞的功能研究[J].2000,(3).
- [2]王月丹,谢玮,邱玉华,等.细胞因子诱导不同来源的贴壁细胞分化为树突状细胞的研究[J].2000,(3).
- [3]张锦堃,陈海滨,孙劲旅,等.GM-CSF和TNF对人血树突状细胞抗肿瘤活性的影响[J].2000,(1).
- [4]陈君敏,陈志哲,张臣青,等.自体树突状细胞活化的骨髓细胞对慢性粒细胞白血病细胞毒性作用研究[J].2001,(2).
- [5]解丽华,王冠军,王晓,等.人脐血CD+34细胞体外诱导树突状细胞及其功能的检测[J].2001,(3).
- [6]刘艳,朱美琪,张弘.脐血中树突状细胞的体外诱导和抗肿瘤效应的实验研究[J].2000,(6).
- [7].人慢性粒细胞白血病树突状细胞的培养和鉴定[J].2000,(5).
- [8]M J, Barnett,C J, Eaves,G L, Phillips,etc.Autografting with cultured marrow in chronic myeloid leukemia: results of a pilot study.[J].1994,84(3).
- [9]B A, Choudhury,J C, Liang,E K, Thomas,etc.Dendritic cells derived in vitro from acute myelogenous leukemia cells stimulate autologous, antileukemic T-cell responses.[J].1999,93.
- [10]S B, Cohen,J A, Madrigal.Immunological and functional differences between cord and peripheral blood.[J].1998,21 Suppl 3.
- [11]G J, Bosch,A M, Joosten,J H, Kessler,etc.Recognition of BCR-ABL positive leukemic blasts by human CD4+ T cells elicited by primary in vitro immunization with a BCR-ABL breakpoint peptide.[J].1996,88.
- [12]朱学军,曹雪涛,马施华,等.人骨髓、脐血CD34~+干细胞体外扩增的树突状细胞的表型及其T细胞刺激活性分析[J].1999,(1).
- [13]王胜军.人树突状细胞体外诱导分化扩增方法[J].2000,(3).
- [14]Liu A,Furukawa T,Aizawa Y,etc.Generation of bcr-abl specific cytotoxic T-lymphocytes by using dendritic cells pulsed with bcr-abl (b3a2) peptide: its applicability for donor leukocyte transfusions in marrow grafted CML patients.[J].1999,13(2).
- [15]A, Choudhury,J L, Gajewski,J C, Liang,etc.Use of leukemic dendritic cells for the generation of antileukemic cellular cytotoxicity against Philadelphia chromosome-positive chronic myelogenous leukemia.[J].1997,89.
- [16]Birgit Herbst,Gabriele Köhler,Andreas Mackensen,etc.CD34+ peripheral blood progenitor cell and monocyte derived dendritic cells: a comparative analysis[J].1997,99(3).
- [17]王晓,裴雪涛,李梁.脐血CD34+细胞和外周血单核细胞两种来源的树突状细胞特性的比较研究[J].1999,(11).