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THE STUDY OF HUMAN ACUTE MYELOID LEUKEMIA CELLS DIFFERENTIATING IN VITRO INTO DENDRITIC CELLS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2003.04.003
- Key Word:
- 树突状细胞;白血病;髓性;急性;细胞因子;混和淋巴细胞反应
Abstract： 目的:探讨利用一定组合的细胞因子,将不同亚型的急性髓性白血病(acute myeloidleukemia,AML)细胞在体外转化为树突状细胞(dendritic cell,DC).方法:8例初发AML患者(其中M11例、M2a 3例、M 32例、M4c1例、M51例)的骨髓单个核细胞(BMMNC),在含GM-CSF、IL-4和TNF-α的完全培养液中培养14天,通过光镜及电镜下观察细胞形态、流式细胞仪测定细胞免疫表型和MTT法混和淋巴细胞反应来鉴定DC.结果:6例患者的BMMNC培养14天后转化为DC.DC的免疫表型CD1.、CD83、CD86、HLA-DR较培养前明显上调(P＜0.05).DC对同种异体T淋巴细胞有较强的刺激作用,远高于对照(P＜0.05),而且随DC数量的增加刺激T细胞增生的活性越强.2例患者(M2a1例,M31例)的BMMNC未转化为DC,免疫表型分析显示CD83及CD1a的表达较低,但CD86及HLA-DR的表达明显增高.结论:利用一定组合的细胞因子可以将AML细胞在体外转化为形态、表型和功能符合DC特征的细胞.某些AML患者的原始细胞不能转化为DC,与其亚型无关,原因有待进一步探讨.
- [1]B A, Choudhury,J C, Liang,E K, Thomas,etc.Dendritic cells derived in vitro from acute myelogenous leukemia cells stimulate autologous, antileukemic T-cell responses.[J].1999,93(3).
- [2]J G, Gribben,E C, Guinan,V A, Boussiotis,etc.Complete blockade of B7 family-mediated costimulation is necessary to induce human alloantigen-specific anergy: a method to ameliorate graft-versus-host disease and extend the donor pool.[J].1996,87.
- [3]N, Romani,S, Gruner,D, Brang,etc.Proliferating dendritic cell progenitors in human blood.[J].1994,180.
- [4]Lowenberg B,Downing JR,Burnett A.Acute myeloid leukemia.[J].1999,341(14).
- [5]Dr. James W. Young,Ralph M. Steinman.The Hematopoietic Development of Dendritic Cells: A Distinct Pathway for Myeloid Differentiation[J].1996,14(4).
- [6]N, Hirano,T, Takahashi,T, Takahashi,etc.Expression of costimulatory molecules in human leukemias.[J].1996,10.
- [7]V A, Boussiotis,G J, Freeman,G, Gray,etc.B7 but not intercellular adhesion molecule-1 costimulation prevents the induction of human alloantigen-specific tolerance.[J].1993,178.
- [8]C L, Law,B, W?rmann,T W, LeBien.Analysis of expression and function of CD40 on normal and leukemic human B cell precursors.[J].1990,4.
- [9]Régis T. Costello,Françoise Mallet,Danielle Sainty,etc.Regulation of CD80/B7-1 and CD86/B7-2 molecule expression in human primary acute myeloid leukemia and their role in allogenic immune recognition[J].1998,28(1).