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THE VALUE OF TRISONY 22 IN THE DETECTION OF INV(16) IN AML
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2003.03.003
- Key Word:
- 白血病;髓细胞;急性;16号染色体倒位;荧光原位杂交;着丝粒
Abstract： 目的:探讨三体22在诊断inv(16)急性髓细胞白血病(AML)中的意义..方法:分别采用Spectrum Green标记的22号染色体着丝粒DNA探针(CEP22),和以红、绿荧光素直接标记的MYH11基因断裂点分开的两侧序列作为探针,对1例伴inv(16)/+22克隆异常的AML及1例单纯+22克隆异常的AML进行单色间期FISH和D-FISH检测,并与细胞形态学及常规细胞遗传学(CC)相比较.结果:2例经采用CEP22探针的FISH技术证明确实存在三体22克隆性异常,而D-FISH均证实inv(16)阳性,其中1例的少数间期核(1.6%)同时伴有16p13的微小缺失.结论:由于+22是一种染色体数目异常,相对来说易于辨认,可以看做是预报inv(16)AML的重要信号,具有潜在的诊断inv(16)AML的价值.
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