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RELATIONSHIP BETWEEN MYELODYSPLASTIC SYNDROMES AND Fas/Fasl SYSTEM AND APOPTOSIS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.06.009
- Key Word:
- 凋亡;Fas(CD95)抗原;骨髓增生异常综合征
Abstract： 目的:研究Fas/Fasl系统及凋亡与骨髓增生异常综合征(myelodysplastic syndrome,MDS)发生发展的关系.方法:分别用TUNEL方法和免疫组化标记方法检测凋亡细胞和Fas、Fasl表达细胞.而后在光镜下计数相应的阳性细胞并计算其百分率.另外,还以ELISA方法检测患者血清中的sFas的含量.结果:(1)MDS组BMMNC中的凋亡细胞百分率[(22.83±10.14)%]高于正常对照组[(2.27±0.74)%](P＜0.05);(2)MDS组BMMNC中的Fas表达细胞百分率[(24.37±6.42)%]高于正常对照组[(1.93±0.32)%](P＜0.05);(3)MDS组BMMNC中的Fasl表达细胞百分率[(22.27±10.50)%]高于正常对照组[(4.18±1.02)%](P＜0.05);(4)MDS组血清sFas的含量[(9.24±10.50)μg/L]高于正常对照组[(7.55±0.79)μg/L](P＜0.05);(5)MDS组凋亡细胞百分率与Fas+细胞或Fasl+细胞百分率之间无直线相关关系(P＞0.05).结论:实验结果表明Fas/Fasl系统介导的凋亡以及机体对其调控的失常参与了MDS骨髓无效造血的病理生理过程.但体内可能还存在另外的机制可引起MDS患者BMMNC凋亡增加.
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