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THE IMMUNOSTIMULATORY EFFECT OF DENDRITIC CELLS BY PULSED WITH CHRONIC MYELOGENOUS LEUKEMIC LYSATE ANTIGEN
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.05.004
- Key Word:
- 树突状细胞;慢性粒细胞白血病;细胞因子诱导的杀伤细胞;免疫治疗
Abstract： 目的:研究慢性粒细胞白血病(CML)细胞冻融抗原(CLA)负载的树突状细胞(DC)对特异性抗白血病T细胞的作用.方法:将健康人外周血单个核细胞来源的DC在体外用CLA负载,再与CML患者的经细胞因子诱导的杀伤细胞(CIK)共同培养,应用乳酸脱氢酶释放法观察其对自身CML细胞杀伤活性,与未负载的DC与CIK共培养组、CIK组分别进行比较.结果:DC经抗原负载后介导的特异性抗白血病T细胞对CML细胞杀伤活性明显增强.结论:用CLA负载可进一步提高DC介导的特异性抗白血病T细胞对CML细胞的杀伤活性.
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