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CLONING OF FUNCTIONAL MACROPHAGE COLONY STIMULATING FACTOR RECEPTOR BARF1 GENE OF EBV FROM J6-1 CELLS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.05.002
- Key Word:
- EB病毒;白血病;病毒感染
Abstract： 目的:从潜伏感染EB病毒的人白血病细胞系J6-1克隆EB病毒功能性巨噬细胞集落刺激因子受体(M-CSFR)基因BARF1.方法:从J6-1细胞提取DNA,利用PCR技术扩增出EB病毒BARF1基因编码区基因片段,连接至载体pMD18-T Vector,并转化Ecoli.对这一克隆片段进行了序列分析.结果:克隆了BARF1基因,证明其与EB病毒B95-8株相关基因同源性为99%,并发现J6-1细胞BARF1编码区第577位出现一个核苷酸的缺失,引起下游序列移码,使编码区提前终止于第597位.结论:上述结果为深入研究BARF1与M-CSF及其受体之间的相互作用奠定了基础,为潜伏感染EB病毒白血病细胞的发病学研究提供了新的线索.
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