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PREPARATION OF MONOCLONAL ANTIBODY TO NUCLEAR MATRIX PROTEIN OF ACUTE MONOCYTIC LEUKEMIA CELLS AND ITS PRELIMINARY IDENTIFICATION

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Author:
No author available
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
3
DOI:
10.3760/cma.j.issn.1009-9921.2001.03.002
Key Word:
核基质;急性单核细胞白血病;单克隆抗体

Abstract: 目的:制备抗急性单核细胞白血病细胞核基质蛋白的特异性单抗.方法:SDS-PAGE分析正常骨髓细胞与急性单核细胞白血病细胞核基质蛋白的差异.用急性单核细胞白血病细胞核基质免疫Balb/c小鼠,利用杂交瘤技术制备了一株能稳定分泌单克隆抗体的杂交瘤细胞株8E7,并对其作了初步鉴定.结果:免疫组化结果显示,8E7单抗与急性单核细胞白血病细胞核仁和胞质反应,与正常骨髓单核细胞胞核及红白血病细胞株K562细胞胞质反应.Western Blotting结果表明,8E7单抗与电泳图谱上急单白血病细胞核基质94 kD的蛋白反应,与正常骨髓细胞核基质27 kD的蛋白反应.结论:对8E7单抗反应蛋白的进一步研究有助于阐明白血病的发病机制.

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