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RESEARCH OF TELOMERASE ACTIVITY AND p53 PROTEIN IN ACUTE LEUKEMIA
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.02.006
- Key Word:
- 白血病;急性;端粒酶;p53
Abstract： 目的:探讨急性白血病(AL)患者中端粒酶活性与突变型p53蛋白的表达规律.方法:采用聚合酶链反应-酶联免疫吸附法(PCR-ELISA)对端粒酶活性进行半定量测定,用流式细胞仪检测突变型p53蛋白表达.结果:AL患者端粒酶活性显著高于对照组(P＜0.001);端粒酶活性在AL的不同病期有统计学差异,其中初治组、复发组均显著高于完全缓解(CR)组(P＜0.01);在治疗敏感组与不敏感组间无统计学差异(P＞0.05).AL患者中突变型p53蛋白阳性率为19.4%(7/36),在AL的不同病期p53阳性率无统计学差别;在初治敏感组与不敏感组之间亦无统计学差别(P均＞0.05).端粒酶活性与突变型p53蛋白的表达无关.结论:端粒酶活性与AL的病期和发展密切相关;突变型p53蛋白在AL中表达较低,对临床预后的判断价值较小;端粒酶的激活与p53蛋白表达无相关性.
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