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THE EXPRESSION OF Ki-67 AND AgNORs IN LEUKEMIA CELLS AND THEIR CLINICAL RELEVANCE
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.01.009
- Key Word:
- 白血病;Ki-67;核仁形成区嗜银蛋白
Abstract： 目的:研究人骨髓白血病细胞中增殖相关抗原Ki-67的表达和核仁形成嗜银蛋白(AgNORs)及其与病程的关系.方法:采用免疫组化ABC法和一步银染技术,分别检测21例正常骨髓和75例白血病骨髓细胞中Ki-67的表达和AgNORs,并动态观察它们在白血病不同阶段的变化.结果:不同类型白血病细胞的Ki-67指数分别为2.5%(AML)、12.2%(ALL)、17.25%(CML),均显著低于正常骨髓原早幼细胞Ki-67指数(23.2%);不同类型白血病的AgNORs分别为3.60±0.63(AML)、3.44±0.58(ALL),均显著高于正常对照组(1.90±0.40);白血病化疗前Ki-67指数高者,缓解率高;白血病达完全缓解后,Ki-67指数和AgNORs接近正常.结论:骨髓原(早)幼细胞Ki-67指数在一定程度上反映了白血病细胞的增殖活性,AgNORs反映了白血病细胞增殖分化不同步现象,二者可用于白血病的病情监测和预后评估.
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