Differential Responses to UVB Irradiation in Human Keratinocytes and Epidermoid Carcinoma Cells
- Author:
- No author available
- Journal Title:
- BIOMEDICAL AND ENVIRONMENTAL SCIENCES
- Issue:
- 5
- DOI:
- 10.3967/0895-3988.2012.05.013
- Key Word:
- UVB;G1‐S phase arrest;HaCaT;A431;NF‐κB;BCL‐2;CDK6;Salidroside
Abstract： Objective To examine UVB‐induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.Methods Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF‐κB, BCL‐2, and CDK6 after 50 J/m2 UVB irradiation were detected by RT‐PCR and western blotting.Results Our results confirmed greater tolerance of A341 cells to UVB‐induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF‐κB,BCL‐2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G1‐S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.Conclusion Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF‐κB, CDK6, and BCL‐2 in UVB‐induced cell G1‐S phase arrest.Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.
- [1]C, Saliou,M, Kitazawa,L, McLaughlin,etc.Antioxidants modulate acute solar ultraviolet radiation-induced NF-kappa-B activation in a human keratinocyte cell line.[J].1999,26(1-2).
- [2]Wu LX,Zhu YZ,Zhong H,etc.Salidroside attenuates apoptosis in ischemic cardiomyocytes: a mechanism through a mitochondria-dependent pathway.[J].2010,114(4).
- [3]Zhao, Y.,Meng, Y.,Zheng, C.,etc.Synthesis, biological activity of salidroside and its analogues[J].2010,58(12).
- [4]G J, Fisher,S C, Datta,H S, Talwar,etc.Molecular basis of sun-induced premature skin ageing and retinoid antagonism.[J].1996,379(6563).
- [5]Denning MF,Nickoloff BJ,Choubey D,etc.Apoptosis in proliferating, senescent, and immortalized keratinocytes.[J].1999,274(33).
- [6]Hart PH,Ng RL,Bisley JL,etc.Ultraviolet irradiation of mice reduces the competency of bone marrow-derived CD11c+ cells via an indomethacin-inhibitable pathway.[J].2010,185(12).
- [7]Streilein RD,Hall RP,Pinnell SR,etc.A topical antioxidant solution containing vitamins C and E stabilized by ferulic acid provides protection for human skin against damage caused by ultraviolet irradiation.[J].2008,59(3).
- [8]Bergstresser PR,Takashima A,Eng W,etc.A role for NF-kappaB-dependent gene transactivation in sunburn.[J].2000,105(12).
- [9]Sontheimer R,Ting WW,Vest CD.Practical and experimental consideration of sun protection in dermatology.[J].2003,42(7).
- [10]Mariarosaria, D'Errico,Massimo, Teson,Angelo, Calcagnile,etc.Differential role of transcription-coupled repair in UVB-induced response of human fibroblasts and keratinocytes.[J].2005,65.
- [11]M, D'Errico,M, Teson,A, Calcagnile,etc.Apoptosis and efficient repair of DNA damage protect human keratinocytes against UVB.[J].2003,10(6).
- [12]Joo CK,Kim JK,Lee DH.Translocation of nuclear factor-kappaB on corneal epithelial cells induced by ultraviolet B irradiation.[J].2005,37(2).
- [13]Pearson AJ,Saunders RD,Priestner MC,etc.Wavelength dependence of cellular responses in human melanocytes and melanoma cells following exposure to ultraviolet radiation.[J].2006,82(11).
- [14]Masaki T,Tokura Y,Bito T,etc.Ultraviolet light induces Stat3 activation in human keratinocytes and fibroblasts through reactive oxygen species and DNA damage.[J].2010,19(7).
- [15]Assefa Z,Van Laethem A,Garmyn M,etc.Ultraviolet radiation-induced apoptosis in keratinocytes: On the role of cytosolic factors[J].2005,1755(2).
- [16]Yu D,Zhang X,Hu X,etc.Salidroside induces cell-cycle arrest and apoptosis in human breast cancer cells.[J].2010,398(1).
- [17]M. D'Errico,M. Teson,A. Calcagnile,etc.Characterization of the ultraviolet B and X-ray response of primary cultured epidermal cells from patients with disseminated superficial actinic porokeratosis[J].2004,150(1).
- [18]Cho TH,Lee MH,Lee JW.Evaluating the cytotoxic doses of narrowband and broadband UVB in human keratinocytes, melanocytes, and fibroblasts.[J].2008,24(3).