Effect of lipid-bound apoA-Ⅰ cysteine mutants upon lipopolysaccharide-induced endotoxemia in mice
- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 44
- DOI:
- 10.3760/cma.j.issn.0376-2491.2009.44.014
- Key Word:
- 脂蛋白类;HDL;载脂蛋白A-Ⅰ;肿瘤坏死因子α;白细胞介素类;Lipoproteins;HDL;ApolipoproteinA-Ⅰ;Tumor necrosis factor-alpha;Interleutins
Abstract： Objective To determine the anti-inflammatory functions of different cysteine mutants of apolipoprotein A-Ⅰ recombinant HDLs. Methods The authors reconstituted recombinant HDLs (namely rHDL74, rHDL129, rHDL195 and rHDL228) by mixing wild type or those mutants with dipalmitoyl phosphatidylcholine and examined their in vivo effects upon LPS-induced endotoxemia in mice. Results At 24 h post-injection, mice receiving rHDL74 [TNF-α: (24±3) pg/ml; IL-1β:(45±5) pg/ml] had a significant decrease of plasma tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) as compared with control mice receiving either saline or rHDLwt [TNF-α: (135±12) pg/ml; IL-1β:(82±8) pg/ml, P < 0.05]. Administration of rHDL74 to mice injected with LPS also led to a protection of lung against acute injury and attenuation of endotoxin-indueed clinical symptoms in mice as compared with controls injected with LPS only. Conclusion Compared with rHDLwt, rHDL74 exhibits higher anti-inflammation capabilities. And it may be a potential clinical candidate for therapy for endotoxin-indueed septic shock.
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