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Expression of calmodulin in the articular process of vertebrae of adolescent idiopathic scoliosis patients
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 29
- DOI:
- 10.3760/j:issn:0376-2491.2006.29.001
- Key Word:
- 脊柱侧凸;青少年;椎关节突关节;钙调蛋白
Abstract： 目的 研究青少年特发性脊柱侧凸(AIS)患者关节突骨质中钙调蛋白表达的差异.方法 研究对象分为AIS组和先天性脊柱侧凸(CS)组,收集AIS组上端椎和顶椎以及CS组融合范围内上端椎的凸凹侧下关节突.使用免疫组化及原位杂交在蛋白质和mRNA两个水平观察钙调蛋白的表达.使用Image-Pro Plus 5.1真彩色病理图像分析系统进行图像分析,对面密度结果进行统计学分析.结果 钙调蛋白在关节突的骨细胞中表达,蛋白质和mRNA两个水平的表达一致:在凸凹侧关节突之间没有显著差异,顶椎同端椎之间没有显著差异(免疫组化结果分别是0.001 898和0.001 454,原位杂交结果分别是0.002 034和0.001 767),AIS组明显低于CS组(免疫组化结果分别是0.001 454和0.005 346,原位杂交结果分别是0.001 767和0.007 621)(P＜0.05).结论 钙调蛋白在AIS患者关节突骨质中的表达异常提示它可能通过直接调控骨质的代谢生长而影响AIS的发生发展.
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