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Correlation between Stat3 signal transduction pathway and expression of cyclooxygenase-2 in colorectal cancer cells
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 41
- DOI:
- 10.3760/j:issn:0376-2491.2005.41.006
- Key Word:
- 结直肠肿瘤;环氧化酶2;转录信号传导子和激活子;固定化蛋白质印迹
Abstract： 目的观察人结直肠恶性肿瘤中信号传导子和激活子3(Stat3)和环氧化酶2 (COX-2)mRNA的表达与结直肠癌病理特征的相关性,明确 Stat3通路对结直肠癌细胞增殖凋亡及COX-2基因表达的影响.方法应用逆转录-聚合酶链反应(RT-PCR)检测350例结直肠癌组织及其邻近正常肠黏膜中Stat3及COX-2 mRNA的表达;同时用免疫组织化学技术(SP法)检测了p-Stat3及COX-2的表达;将Stat3上游激酶JAK2抑制剂AG490,作用于结肠癌细胞系HT-29,运用四甲基偶氮唑蓝(MTT)法检测细胞增殖状态;流式细胞仪观察其对细胞凋亡的影响,Western 印迹法检测p-Stat3及COX-2蛋白的表达.结果结直肠癌组织中Stat3和COX-2 mRNA表达水平明显高于正常肠黏膜(均P<0.05),分别为正常组织的1.97和1.88倍; COX-2表达水平与Stat3表达呈正相关(相关系数0.749,P<0.01),并与肿瘤分化程度及淋巴结转移有关(P<0.05);免疫组化结果示p-Stat3与COX-2蛋白表达水平相关.用(AG490)阻断Stat3通路可抑制HT-29细胞的增殖、诱导其凋亡; 同时Stat3磷酸化活性随作用时间延长而下降,COX-2表达水平也逐渐降低.结论 Stat3通路对结直肠癌细胞增殖凋亡及COX-2基因表达有影响;阻断信号通路可抑制结肠癌细胞的增殖,促进其凋亡.
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