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Biological effects of extracellular ground substance on bone marrow mesenchymal stem cells
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- Author:
- No author available
- Journal Title:
- CHINA JOURNAL OF MODERN MEDICINE
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1005-8982.2005.11.011
- Key Word:
- 骨髓间冲质干细胞;细胞外基质;分离培养
Abstract： 目的观察细胞外基质对MSCs贴壁、增殖和分化的影响,以期为MSCs建立一种更为理想的培养体系.方法用贴壁法分离纯化大鼠骨髓MSCs,所用培养皿预先用细胞外基质(明胶)处理过夜,以普通培养皿做对照,镜下观察细胞的生物学形态,计数细胞贴壁数目并描绘细胞生长曲线,观察细胞外基质对MCSs生长的影响.结果用细胞外基质处理过的培养皿MSCs,细胞的贴壁、增殖和分化速度都较普通组快,且细胞形态、活力和长势较普通组活跃.结论细胞外基质能明显促进MSCs的分离纯化,使体外扩增培养MSCs更为简单高效.
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