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Detction of foot-and-month disease virus by an ultra-violet spectrophotometry
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1673-4696.2000.11.008
- Key Word:
- 口蹄疫病毒;负相关;置信度
Abstract： 用紫外分光光度计测试口蹄疫病毒(FMDV)含量中,比较研究了PBS、Tris、含不同浓度蔗糖液和不同浓度迭氮钠液的差异性、相关性、直线回归方程及不同置信度下的方程误差.结果表明,蔗糖浓度与FMDV的吸光度值之间有显著直线负相关,以PBS和Tris为对照检测FMDV,对检测结果的不利影响甚微;在FMDV中加迭氮钠的最大量不能大于5.4 mg/L,否则无法测定FMDV的吸光度值;检测蔗糖密度梯度离心纯化的FMDV抗原,必须以相应级份的蔗糖液为对照才能准确定量.
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