Development and application of animal cell culture medium
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF CLINICAL REHABILITATION
- Issue:
- 41
- DOI:
- 10.3321/j.issn:1673-8225.2006.41.063
- Key Word:
- 培养基;无血清;细胞;培养的;综述文献
Abstract： 目的:动物细胞培养基是体外细胞培养的重要因素,能够影响细胞生长.为此本文就动物细胞培养基的发展及应用作以系统的总结.资料来源:应用计算机检索PUBMED 1990-01/2006-03期间的相关文章,检索词为"cell culture,culture medium,serum-free medium,development",并限定文章语言种类为英文.同时计算机检索万方数据库1990-01/2006-03期间的相关文章,检索词为"细胞培养,培养基,无血清培养基,发展",并限定文章语言种类为中文.资料选择:对资料进行初审,并查看每篇文献后的引文.纳入标准:文章所述内容应与动物细胞培养基的发展及应用研究相关.排除标准:重复研究或Meta分析类文章.资料提炼:共收集到63篇相关文献.其中28篇文献符合纳入标准,排除的35篇文献为内容陈旧或重复.符合纳入标准的28篇文献中,18篇涉及动物细胞培养基发展的3个阶段及其应用,4篇涉及无血清培养基的分代,6篇涉及无血清培养基的应用.资料综合:动物细胞培养基划分为天然培养基、合成培养基和无血清培养基3个阶段:天然培养基阶段是对动物细胞培养基最初的也是最原始的研究阶段,为合成培养基阶段的到来打下了坚固的基础.合成培养基阶段在天然培养基阶段的基础上发展较为迅速,可以通过添加某些成分支持细胞增殖.小牛血清成为合成培养基最常用的添加成分,虽然小牛血清中含有很多支持细胞生长的活性物质但同时也给细胞培养造成了诸多的不利.无血清培养基阶段旨在寻求血清的替代成分,既能满足细胞培养的要求又能避免血清引起的一系列问题.无血清培养基的发展共分为4代,前3代存在很多缺点如适用谱窄,培养基难以保存、容易受到外界因素的影响等.目前正致力于一种既无血清,无蛋白,又可以高温消毒的适合于许多种不同细胞生长的全能型培养基的研究与开发.希望通过对动物细胞培养基的逐步研究完善动物细胞培养技术,使其在生物制品的制备和基因工程领域得到更广泛的应用.结论:在动物细胞培养基的各个发展阶段,无血清培养基均存在明显优势,具有广泛的应用前景.
- [1]黄斌,牛红星,朱明龙,等.rCHO细胞无血清适应及悬浮培养[J].2004,(1).
- [2]朱宏庆,周燕,华平,等.MCDB153无血清培养基的优化[J].2005,(3).
- [3]王祥斌,孔健.体外培养杂交瘤细胞生产人用鼠源单克隆抗体[J].2002,(5).
- [4]Ru Zhang,Jun Gong,Hui WANG,etc.Bile salts inhibit growth and induce apoptosis of culture human normal esophageal mucosal epithelial cells[J].2005,(41).
- [5]姜明,史俊南,荫俊.一种合成肽增强多黏菌素B中和内毒素的能力[J].2003,(6).
- [6]孙红,车鹏程,黑砚,等.两种不同培养体系培养人角朊细胞的特征[J].2005,(2).
- [7]邹寿长,李干祥,杨葆生,等.大规模动物细胞培养技术研究进展[J].2001,(2).
- [8]魏寿江,王崇树,王严庆,等.重组人生长激素对体外人结肠癌COLO-320细胞生长的影响[J].2003,(6).
- [9]王京华,郭搏,刘芳,等.多发性骨髓瘤与正常人来源树突状细胞培养及特性的比较研究[J].2004,(4).
- [10]Uriel, Elchalal,W Timothy, Schaiff,Steven D, Smith,etc.Insulin and fatty acids regulate the expression of the fat droplet-associated protein adipophilin in primary human trophoblasts.[J].2005,193(5).
- [11]赵佼,谭文松,俞俊棠.动物细胞培养工程的现状与展望[J].1997,(2).
- [12]Sinacore Martin S..Adaptation of Mammalian Cells to Growth in Serum-Free Media[J].2000,15(3).
- [13]Asakawa T,Tomioka T,Kanematsu T.A method for culturing and transplanting biliary epithelial cell from syrian golden hamster.[J].2000,436(2).
- [14]Tomasz, Drewa,Bartosz, Nadolski,Artur, Czaplewski,etc.[Application of autologous and allogenic keratynocyte cultures in burn management].[J].2005,62.
- [15]忻亚娟.动物细胞培养技术的进展[J].2001,012 (4).
- [16]Kessler W,Hesse F,Ebel M,etc.Comparison of a production process in a membrane-aerated stirred tank and up to 1000-L airlift bioreactors using BHK-21 cells and chemically defined protein-free medium[J].2003,19(3).
- [17]A, Okanlawon,O, Kusemiju,M, Dym.Direct toxic effects of clinical doses of chloroquine on transferrin secretion in immature rat sertoli cells in vitro.[J].2001,8(4).
- [18]C, De Bruyn,A, Delforge,D, Bron.Ex vivo myeloid differentiation of cord blood CD34+ cells: comparison of four serum-free media containing bovine or human albumin.[J].2003,5(2).
- [19]Erika Spens,Lena Haggstrom.Defined Protein-Free NSO Myeloma Cell Cultures:Stimulation of proliferation by Conditioned Medium Factors[J].2005,21(1).
- [20]陈昭烈,肖成祖.动物细胞无血清培养基及其应用[J].1994,(5).
- [21]J. H. Ryu,S.-S. Kim,S.-W. Cho.HEK 293 Cell Suspension Culture Using Fibronectin-Adsorbed Polymer Nanospheres in Serum-Free Medium[J].2004,71A(1).
- [22]J, Mao,M F, Smith,E B, Rucker,etc.Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro.[J].2004,82(7).