You Position: Home > Paper
Effect of cigarette smoking on macrophage cholesterol efflux mediated by mice serum and the potential mechanisms
( views:198, downloads:0 )
- Author:
- No author available
- Journal Title:
- PROGRESS IN MODERN BIOMEDICINE
- Issue:
- 23
- DOI:
- No doi available
- Key Word:
- 吸烟;巨噬细胞;胆固醇流出;动脉粥样硬化;高密度脂蛋白
Abstract： 目的:探讨吸烟对小鼠血清介导巨噬细胞胆固醇流出的影响及其机制.方法:14只C57BL/6小鼠随机分为对照组和吸烟组.吸烟组小鼠被动吸烟8周后,检测血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)和丙二醛(MDA)浓度.采用液体闪烁计数法分别检测对照组小鼠血清、吸烟组小鼠血清、吸烟组小鼠血清+N-乙酰半胱氨酸(NAC,2mM)介导的细胞胆固醇流出效率.结果:与对照组相比,吸烟组小鼠血清中HDL-C显著降低,TG显著升高,TC和LDL-C无显著差别,并且吸烟组小鼠血清中MDA水平也显著升高.吸烟组小鼠血清介导巨噬细胞胆固醇流出效率明显降低,给予抗氧化剂NAC共同孵育可以显著增加吸烟组小鼠血清胆固醇流出效率.结论:吸烟显著降低小鼠血清介导的巨噬细胞胆固醇流出效率,其机制可能与吸烟降低HDL-C水平以及促进HDL脂质过氧化有关.
- [1]H S, Kruth.Macrophage foam cells and atherosclerosis.[J].2001,6.
- [2]Joaquin, Barnoya,Stanton A, Glantz.Cardiovascular effects of secondhand smoke: nearly as large as smoking.[J].2005,111(20).
- [3]Ansell BJ,Fonarow GC,Fogelman AM.The paradox of dysfunctional high-density lipoprotein.[J].2007,18(4).
- [4]Ansell BJ,Singh IM,Shishehbor MH.High-density lipoprotein as a therapeutic target: a systematic review.[J].2007,298(7).
- [5]Daniel J. Rader,George H. Rothblat,Eric T. Alexander,etc.The role of reverse cholesterol transport in animals and humans and relationship to atherosclerosis[J].2009,50(S.1959).
- [6]Kazuhisa Ueyama,Masayuki Yokode,Hidenori Arai,etc.Cholesterol Efflux Effect of High Density Lipoprotein is Impaired by Whole Cigarette Smoke Extracts Through Lipid Peroxidation[J].1998,24(1).
- [7]Baldassarre D,Castelnuovo S,Frigerio B,etc.Effects of timing and extent of smoking, type of cigarettes, and concomitant risk factors on the association between smoking and subclinical atherosclerosis.[J].2009,40(6).
- [8]McGillicuddy FC,Billheimer JT,Reilly MP,etc.Inflammation impairs reverse cholesterol transport in vivo.[J].2009,119(8).
- [9]Craig W. Y.,Palomaki G. E.,Haddow J. E..Cigarette smoking and serum lipid and lipoprotein concentrations: an analysis of published data.[J].1989.
- [10]Cai S,Chen JB,Chen P,etc.Oral N-acetylcysteine attenuates pulmonary emphysema and alveolar septal cell apoptosis in smoking-induced COPD in rats.[J].2009,14(3).