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Diagnostic and clinical significance of detecting bcr-abl gene in chronic myeloid leukemia
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- Author:
- No author available
- Journal Title:
- SHANDONG MEDICAL JOURNAL
- Issue:
- 44
- DOI:
- 10.3969/j.issn.1002-266X.2009.44.007
- Key Word:
- 白血病;粒细胞;慢性;实时定量PCR;bcr-abl融合基因
Abstract： 目的 探讨定量检测ber-abl融合基因在慢性粒细胞白血病(CML)诊断、治疗及微小残留病变监测中的意义.方法 应用实时定量PCR(RQ-PCR)方法检测145例CML患者bcr-abl融合基因的表达情况.结果 abl和bcr-abl的标准曲线相关系数均大于0.99,批内差及批间差均小于4%;11例不同分期的CML患者同时检测外周血和骨髓中bcr-abl水平,8例外周血较骨髓低,3例较骨髓高;急变期的bcr-abl表达情况较加速期和慢性期均明显增高.结论 RQ-PCR技术检测bcr-abl融合基因准确可靠,对于评价疗效、监测微小残留病变以及预测疾病进展具有重要的临床应用价值.
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