You Position: Home > Paper
Effects of Quercetin on telomerase activity and apoptosis in gastric cancer cells
( views:188, downloads:8 )
- Author:
- No author available
- Journal Title:
- SHANDONG MEDICAL JOURNAL
- Issue:
- 35
- DOI:
- 10.3969/j.issn.1002-266X.2007.35.005
- Key Word:
- 胃肿瘤;槲皮素;端粒酶;凋亡
Abstract： 目的 观察槲皮素对胃癌细胞增殖的影响,并探讨可能的作用机制.方法 采用不同浓度的槲皮素处理胃癌SGC7901细胞,以四氮唑盐(MTT)比色试验观察胃癌细胞增殖情况,以TRAP-ELISA方法检测胃癌细胞端粒酶活性,分别采用琼脂糖凝胶电泳法和TUNEL法检测胃癌细胞凋亡情况.结果 槲皮素处理后胃癌SGC7901细胞增殖率及细胞端粒酶活性明显降低、细胞凋亡率增加,且呈浓度和时间依赖性(P《0.05).结论 槲皮素可抑制胃癌细胞增殖,可能机制为抑制端粒酶活性和诱导细胞凋亡.
- [1]范钰,林庚金.端粒及端粒酶与胃粘膜癌变诊疗的研究进展[J].2001,(6).
- [2]林庚金,范钰,钱立平,等.大鼠胃黏膜癌变过程细胞凋亡及相关癌基因bcl-2、c-myc的动态表达[J].2001,(7).
- [3]YU Fan,Shu Zheng,Ze-Feng Xu,etc.Apoptosis induction with polo-like kinase-1 antisense phosphorothioate oligodeoxynucleotide of colon cancer cell line SW480[J].2005,(29).
- [4]范钰,郑树,丁佳逸.脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性[J].2006,(4).
- [5]Hollman,PC,Katan,MB.Dietary flavonoids: intake, health effects and bioavailability.[J].1999,37(9/10).
- [6]Shiota G,Oshimura M,Kawasaki H,etc.Clinical usefulness of telomerase activity and telomere length in the preoperative diagnosis of gastric and colorectal cancer.[J].1999,125(7).
- [7]N W, Kim,M A, Piatyszek,K R, Prowse,etc.Specific association of human telomerase activity with immortal cells and cancer.[J].1994,266(5193).