You Position: Home > Paper
Significance of T-bet/GATA-3 ratio in immune disbalance of asthmatic rats
( views:165, downloads:9 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2006.18.017
- Key Word:
- 哮喘;转录因子;T淋巴细胞
Abstract： 目的:探讨转录因子T-bet/GATA-3比值在支气管哮喘免疫失衡中的意义.方法:SPF级SD大鼠随机分为对照组和哮喘组,每组12只.用逆转录-聚合酶链反应(RT-PCR)测定法和Western blot法分别检测T-bet,GATA-3 mRNA和蛋白在肺组织的表达.结果:大鼠肺组织中对照组和哮喘组T-bet mRNA表达分别为0.71±0.19,0.08±0.12;T-bet蛋白表达分别为0.72±0.22,0.18±0.06,两组比较均有显著性差异(P＜0.01).大鼠肺组织中对照组和哮喘组GATA-3 mRNA表达分别为1.06±0.25,1.56±0.28;GATA-3蛋白分别为1.04±0.44,2.25±0.74,两组比较均有显著性差异(P＜0.01);对照组T-bet/GATA-3 mRNA表达量的比值0.73±0.32,明显高于哮喘组0.06±0.09 (P＜0.01),对照组T-bet/GATA-3 蛋白表达量的比值0.75±0.25,高于哮喘组0.09±0.04 (P＜0.01).结论:转录因子T-bet/GATA-3 mRNA和蛋白表达量比值在哮喘中明显降低,可作为评价哮喘免疫失衡的客观指标之一.
- [1]刘春涛,王刚,罗凤鸣,等.支气管哮喘患者Th2细胞过度分化与转录因子T-bet和GATA-3的调控作用[J].2004,(6).
- [2]吕国平,崔德健,郭英江,等.介绍一种建立大鼠哮喘模型的实验方法[J].1995,(6).
- [3]De-Sanctis GT,Bartsch B,Schipp M,etc.Treatment of allergic airway inflammation and hyperresponsiveness by antisense-induced local blockade of GATA-3 expression.[J].2001,193(11).
- [4]S J, Szabo,S T, Kim,G L, Costa,etc.A novel transcription factor, T-bet, directs Th1 lineage commitment.[J].2000,100(6).
- [5]Ian R, Rifkin,Ann, Marshak-Rothstein.T-bet: the Toll-bridge to class-switch recombination?[J].2003,4(7).
- [6]Lee HJ,Arai N,Takemoto N,etc.GATA-3 induces T helper cell type 2 (Th2) cytokine expression and chromatin remodeling in committed Th1 cells.[J].2000,192(1).
- [7]Lefebvre DE,Chakir H,Webb J,etc.T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3.[J].2003,278(1).
- [8]W, Zheng,R A, Flavell.The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells.[J].1997,89(4).