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Effects of indole-3-acetic acid oxidated by horseradish peroxidase on proliferation and MDA level of HeLa cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2004.17.013
- Key Word:
- 吲哚乙酸;辣根过氧化物酶;HeLa细胞;增殖;丙二醛;凋亡
Abstract： 目的:研究辣根过氧化物酶(horseradish peroxidase, HRP)激活吲哚乙酸(indoleacetic acid,IAA)后产生的细胞毒性作用对HeLa细胞增殖及丙二醛(malondialdehyde,MDA)水平的影响. 方法:取不同处理时间点体外培养的人宫颈癌HeLa细胞,经不同终浓度的IAA(分别为20,40,60,80,100 μmol/L)和同一终浓度的1.2 mg/L HRP共同处理后,依次用台盼蓝拒染法、生长抑制实验、集落形成实验、流式细胞术、TBA法及QWin550CW显微镜,检测活细胞数、抑制率、集落形成率、增殖指数、凋亡率和MDA含量,观察细胞形态学变化. 结果:HRP激活的IAA对HeLa细胞生长及降解MTT的能力均有较强的抑制作用,在一定的浓度范围内,呈现明显的时效和量效关系. 在6, 24, 48和72 h检测细胞周期,处理组随着IAA浓度增加,细胞凋亡率增加,S期和G2/M期细胞比率增加,G0/G1期细胞比率下降. 随着IAA浓度的增加,HeLa细胞集落生成率逐渐减少,集落体积变小,各处理组蛋白含量显著降低,MDA含量增加,细胞形态学的变化在72 h最明显. 结论:HRP激活的IAA可能通过促进细胞进入分裂期,同时加速脂质过氧化程度的机制来诱导HeLa细胞凋亡.
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