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Effect of hepatitis B virus X gene and hepatitis C virus C gene coexpression protein on telomerase activity of HepG2 cells
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- Author:
- No author available
- Journal Title:
- ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-5404.2005.14.006
- Key Word:
- 乙型肝炎病毒;丙性肝炎病毒;X基因;核心基因;端粒酶
Abstract： 目的建立HBV X-HCV C融合蛋白细胞表达模型,并探讨其对细胞端粒酶活性的影响.方法双酶切质粒pXT1-X,得到完整的HBV X基因片段后,将其插入到质粒PBK-CMV和PBK-HCV C的相应酶切位点,得到重组质粒PBK-X和PBK-X-C;再将质粒PBK-CMV、PBK-X、PBK-HCV C和PBK-X-C分别导入肝癌细胞株HepG2中,G418筛选,RT-PCR、蛋白印迹鉴定HBV X和HCV C蛋白表达.PCR-ELISA法检测端粒酶活性.结果质粒PBK-CMV、PBK-X、PBK-HCV C和PBK-X-C在HepG2细胞中有稳定表达.表达融合蛋白的细胞的端粒酶活性较转染空载体的细胞及单独表达HBV X、HCV C蛋白的细胞明显升高.结论HBV X-HCV C融合蛋白能显著上调端粒酶活性,提示HBV、HCV可能具有协同致癌作用.
- [1]王春艳,王凯,冯冬梅.PCR法与ELISA法检测丙型肝炎病毒及乙型肝炎病毒比较[J].2002,(4).
- [2]刘重阳,刘为纹,杨建民,等.HCV核心基因cDNA真核表达载体的构建及其表达[J].2000,(9).
- [3]闵军,陈亚进,王金林,等.肝癌细胞端粒酶激活的可能机制：乙型肝炎病毒X基因-端粒酶反转录酶途径[J].2001,(6).
- [4]沈钦海,周卫平,张大志,等.乙型肝炎病毒及其X基因对培养肝细胞端粒酶活性的影响[J].2002,(2).
- [5]刘重阳,刘为纹,杨建民,等.丙型肝炎病毒核心蛋白在HepG2细胞中的表达及其对端粒酶活性的影响[J].2002,(4).
- [6](美)萨姆布鲁克(Sambrook,J.)等著 金冬雁.分子克隆实验指南[M].1992.
- [7]Geller SA.Hepatitis B and hepatitis C.[J].2002,6(2).
- [8]Grandori C,Lingner J,Amacker M,etc.Direct activation of TERT transcription by c-MYC.[J].1999,21(2).
- [9]Kim, Nam W.,Piatyszek, Mieczyslaw A..Specific association of human telomerase activity with immortal cells and cancer.[J].1994,266(5193).
- [10]M, Takakura,S, Kyo,T, Kanaya,etc.Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells.[J].1999,59(3).
- [11]Negorev D,Livezey KW,Simon D.Increased chromosomal alterations and micronuclei formation in human hepatoma HepG2 cells transfected with the hepatitis B virus HBX gene.[J].2002,505(1).
- [12]Tsutsumi T,Ishibashi K,Matsuura Y,etc.The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice.[J].1998,4(9).
- [13]Deng H,Hann SR,Greenberg RA,etc.Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation.[J].1999,18(5).
- [14]Alberti A,Benvegnu L.Patterns of hepatocellular carcinoma development in hepatitis B virus and hepatitis C virus related cirrhosis.[J].2001,52(2).