You Position: Home > Paper
Preparation and cryopreservation of dendritic cell vaccine for clinical use
( views:197, downloads:6 )
- Author:
- No author available
- Journal Title:
- ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-5404.2004.14.027
- Key Word:
- 树突状细胞;胃癌;疫苗
Abstract： 目的探讨在体外制备可供临床使用的肿瘤树突状细胞疫苗的有效途径及冻存方法.方法采用外周血单核细胞诱导DCs,自体胃癌细胞裂解抗原负载DCs,制备树突细胞疫苗.分别观察、检测了新鲜制备的DCs疫苗和冻存2周及2月的DCs疫苗的大体形态,免疫表型及刺激同种异体T淋巴细胞增殖的能力,并行细菌学检查.结果新鲜制备的DCs疫苗高表达成熟DCs表面标志物HLA-DR、CD80及CD83,具有很强的刺激同种异体T淋巴细胞增殖的能力.疫苗冻存2个月以内,DCs细胞表型及刺激同种异体T淋巴细胞增殖的能力无明显下降.结论采用本方法制备的DCs疫苗,无论从数量及质量均达到了临床应用的要求.并且证实疫苗冻存2月以内对疫苗质量无明显影响.
- [1]雷晓,余佩武,石彦,等.树突状细胞肿瘤疫苗诱导抗胃癌作用的实验研究[J].2002,(1).
- [2]吴淼,余佩武,蔡志民,等.负载胃癌酸洗抗原树突状细胞诱导高效特异的抗胃癌细胞效应[J].2002,(10).
- [3]Gluck S,Syme R.Effects of cytokines on the culture and differentiation of dendritic cells in vitro.[J].2001,10(1).
- [4]Fields RC,Shimizu K,Mule JJ.Murine dendritic cells pulsed with whole tumor lysates mediate potent antitumor immune responses in vitro and in vivo.[J].1998,95(16).
- [5]A, Curti,M, Fogli,M, Ratta,etc.Dendritic cell differentiation from hematopoietic CD34+ progenitor cells.[J].1900,15(1).
- [6]Wu L,Vandenabeele S,Georgopoulos K.Derivation of dendritic cells from myeloid and lymphoid precursors.[J].2001,20(1).
- [7]Engleman EG.Dendritic cell-based cancer immunotherapy.[J].2003,30(3 Suppl.8).
- [8]J, Banchereau,A K, Palucka,M, Dhodapkar,etc.Immune and clinical responses in patients with metastatic melanoma to CD34(+) progenitor-derived dendritic cell vaccine.[J].2001,61(17).